乙型肝炎病毒粒子DNA在大肠杆菌中的克隆及其物理结构
Cloning in Escherichia coli and physical structure of hepatitis B virion DNA.
作者信息
Charnay P, Pourcel C, Louise A, Fritsch A, Tiollais P
出版信息
Proc Natl Acad Sci U S A. 1979 May;76(5):2222-6. doi: 10.1073/pnas.76.5.2222.
Abstract
A restriction map of hepatitis B virion DNA was established after cloning of the whole viral genome in Escherichia coli. By use of EcoRI, Xho I, Bgl II, Xba I, BamHI, HincII, and Hae III endonucleases, a total of 28 restriction sites were mapped. The single-stranded region was localized on the restriction map and 5' end of the short strand was mapped at a fixed position.
摘要
在将乙肝病毒全基因组克隆到大肠杆菌中后，构建了乙肝病毒颗粒DNA的限制酶切图谱。使用EcoRI、Xho I、Bgl II、Xba I、BamHI、HincII和Hae III核酸内切酶，共定位了28个限制酶切位点。单链区域在限制酶切图谱上定位，短链的5'端在一个固定位置定位。
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