Zinder N D, Boeke J D
Gene. 1982 Jul-Aug;19(1):1-10. doi: 10.1016/0378-1119(82)90183-4.
Derivatives of filamentous phage, f1, fd, and M13, useful as cloning vectors are listed, and procedures for their use are reviewed. Methods for growing phage, preparing single- and double-stranded DNA, and cloning are given in the "cook-book" form. These procedures minimize the practical problem often associated with filamentous-phage cloning, i.e., deletion of inserts.
列出了用作克隆载体的丝状噬菌体f1、fd和M13的衍生物，并综述了它们的使用方法。以“烹饪书”的形式给出了培养噬菌体、制备单链和双链DNA以及克隆的方法。这些方法将丝状噬菌体克隆中经常出现的实际问题，即插入片段的缺失，降到了最低限度。
