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丝状噬菌体(Ff)作为重组DNA载体的综述

The filamentous phage (Ff) as vectors for recombinant DNA--a review.

作者信息

Zinder N D, Boeke J D

出版信息

Gene. 1982 Jul-Aug;19(1):1-10. doi: 10.1016/0378-1119(82)90183-4.

Abstract

Derivatives of filamentous phage, f1, fd, and M13, useful as cloning vectors are listed, and procedures for their use are reviewed. Methods for growing phage, preparing single- and double-stranded DNA, and cloning are given in the "cook-book" form. These procedures minimize the practical problem often associated with filamentous-phage cloning, i.e., deletion of inserts.

摘要

列出了用作克隆载体的丝状噬菌体f1、fd和M13的衍生物,并综述了它们的使用方法。以“烹饪书”的形式给出了培养噬菌体、制备单链和双链DNA以及克隆的方法。这些方法将丝状噬菌体克隆中经常出现的实际问题,即插入片段的缺失,降到了最低限度。

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