Winship T R, Fong C K, Hsiung G D
J Interferon Res. 1983;3(1):71-4. doi: 10.1089/jir.1983.3.71.
Primary guinea pig embryo (GPE) fibroblasts were assessed as potential sources of guinea pig interferon (IFN). GPE cells proved to be excellent in vitro producers of guinea pig IFN, although the actual amounts produced were only detectable when sample irradiation under ultraviolet light (to inactivate inducing viruses) was substituted for overnight sample treatment at pH 2. Thus, the rapid spontaneous inactivation of large proportion of the antiviral activity after overnight exposure to 4 degrees C, regardless of pH, was avoided. IFN was induced using Newcastle disease virus (NDV), Sindbis virus, and a genetic variant of vesicular stomatitis virus, VSV T1026R1. Each virus exhibited different dose response kinetics, with VSV T1026R1 proving the most efficient inducer of the three. Optimal IFN production depended largely on virus multiplicity and cell age. All the antiviral activity produced by GPE fibroblasts had the classical properties of species specificity, susceptibility to trypsin, and a broad range of antiviral activity.
原代豚鼠胚胎（GPE）成纤维细胞被评估为豚鼠干扰素（IFN）的潜在来源。GPE细胞被证明是体外产生豚鼠IFN的优秀细胞，尽管只有在用紫外线照射样品（以灭活诱导病毒）代替在pH 2下过夜处理样品时，才能检测到实际产生的量。因此，避免了在4℃下过夜暴露后，无论pH如何，大部分抗病毒活性迅速自发失活的情况。使用新城疫病毒（NDV）、辛德毕斯病毒和水疱性口炎病毒的遗传变体VSV T1026R1诱导IFN。每种病毒表现出不同的剂量反应动力学，其中VSV T1026R1是三种病毒中最有效的诱导剂。最佳IFN产生在很大程度上取决于病毒复数和细胞年龄。GPE成纤维细胞产生的所有抗病毒活性都具有种属特异性、对胰蛋白酶敏感以及广泛的抗病毒活性等经典特性。
