Elder J K, Southern E M
Anal Biochem. 1983 Jan;128(1):227-31. doi: 10.1016/0003-2697(83)90369-x.
Relationships between DNA length and electrophoretic mobility in an agarose gel have been compared by estimating the lengths of known DNA polymer fragments, using other fragments in the series as standards. Global estimates were made using 10 fragments as standards; local estimates were made using the two closest fragments on either side of the unknown as standards. Most relationships were fitted by least squares. All the relationships gave more accurate local than global estimates. The most accurate results were obtained using the reciprocal relationship, where the maximum error in the local estimates was less than 0.1%. The semilog relationship gave the least accurate results, with a maximum error in the local estimates of almost 5%. The polymer fragments were also used as standards to estimate the lengths of lambda DNA restriction fragments. Here the estimates were in error by up to 3%, indicating the influence of base composition and sequence on electrophoretic mobility.
通过估算已知DNA聚合物片段的长度，并以该系列中的其他片段作为标准，比较了琼脂糖凝胶中DNA长度与电泳迁移率之间的关系。使用10个片段作为标准进行整体估算；使用未知片段两侧最接近的两个片段作为标准进行局部估算。大多数关系采用最小二乘法拟合。所有关系给出的局部估算都比整体估算更准确。使用倒数关系获得了最准确的结果，局部估算中的最大误差小于0.1%。半对数关系给出的结果最不准确，局部估算中的最大误差几乎达到5%。聚合物片段还被用作标准来估算λDNA限制性片段的长度。此处估算的误差高达3%，表明碱基组成和序列对电泳迁移率的影响。
