Malpièce Y, Michel M L, Carloni G, Revel M, Tiollais P, Weissenbach J
Nucleic Acids Res. 1983 Jul 11;11(13):4645-54. doi: 10.1093/nar/11.13.4645.
The properties of the promoter of the hepatitis B surface antigen (HBsAg) were studied using recombinants containing either this promoter or the SV40 early promoter. Mouse L cells were transfected with these recombinants and the levels of gene expression obtained with the two promoters were compared. The level of expression of a cellular gene, the human fibroblast interferon gene, obtained with the HBsAg promoter was comparable to that obtained with the SV40 early promoter. Similarly when the HBsAg gene was controlled by the SV40 early promoter the level of HBsAg synthesis is in the same range as that observed with its own promoter. Together these results suggest that although the HBsAg gene codes for a structural viral protein, its expression is constitutive as for an early gene. The implications of these observations on the synthesis of HBV particles in vivo are discussed.
利用含有乙肝表面抗原（HBsAg）启动子或SV40早期启动子的重组体，对HBsAg启动子的特性进行了研究。用这些重组体转染小鼠L细胞，并比较了两种启动子获得的基因表达水平。用HBsAg启动子获得的细胞基因——人成纤维细胞干扰素基因的表达水平，与用SV40早期启动子获得的表达水平相当。同样，当HBsAg基因由SV40早期启动子控制时，HBsAg的合成水平与其自身启动子所观察到的水平处于同一范围。这些结果共同表明，尽管HBsAg基因编码一种病毒结构蛋白，但其表达与早期基因一样是组成型的。讨论了这些观察结果对体内乙肝病毒颗粒合成的影响。
