通过细胞离心涂片增强直接免疫荧光法检测临床标本中的单纯疱疹病毒
Detection of herpes simplex virus in clinical specimens by cytospin-enhanced direct immunofluorescence.
作者信息
Landry M L, Ferguson D, Wlochowski J
机构信息
Clinical Virology Laboratory, Yale New Haven Hospital, Connecticut, USA.
出版信息
J Clin Microbiol. 1997 Jan;35(1):302-4. doi: 10.1128/jcm.35.1.302-304.1997.
Abstract
For 275 samples tested for herpes simplex virus (HSV), cytospin-enhanced direct immunofluorescence using Chemicon HSV monoclonal antibodies identified 80 (95%) and culture identified 77 (92%) of 84 confirmed positive specimens. Cytospin-prepared slides contained a greater number of total cells than standard cell spots, resulting in fewer inadequate cell smears and a higher HSV detection rate.
摘要
对275份检测单纯疱疹病毒（HSV）的样本而言，使用Chemicon HSV单克隆抗体进行的细胞离心涂片增强直接免疫荧光法在84份确诊阳性标本中识别出80份（95%），而培养法识别出77份（92%）。细胞离心涂片制备的玻片所含的细胞总数比标准细胞涂片多，从而减少了不合格细胞涂片的数量，并提高了HSV检测率。
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