Rogers B B, Josephson S L, Mak S K
Department of Pathology, Women and Infants' Hospital, Providence, Rhode Island 02905.
Am J Pathol. 1991 Jul;139(1):1-6.
The polymerase chain reaction (PCR) was used to amplify herpes simplex virus DNA using a single set of primers that amplify both herpes simplex virus I (HSVI) and II (HSVII). The viruses can be differentiated by a single restriction enzyme cleavage. Virus from dilutions of HSV-infected A549 cell suspensions were amplified and the infectivity endpoints of cell culture were compared with the PCR, and with another direct detection method, the enzyme-linked immunosorbent assay (ELISA). The PCR was capable of detecting virus at a 10(-4) dilution for both HSVI and HSVII, when the corresponding TCID50 endpoints were 10(-5.9) and 10(-5.7), respectively. The ELISA detected virus only down to the 10(-1) dilution. The amplification procedure showed the greatest sensitivity when an initial protease digestion was followed by filtration. The PCR may have use in detection of HSV in clinical situations in which a rapid result is desirable.
聚合酶链反应（PCR）用于使用一组引物扩增单纯疱疹病毒DNA，该引物可同时扩增单纯疱疹病毒I型（HSV I）和II型（HSV II）。这两种病毒可通过单一限制酶切割进行区分。对HSV感染的A549细胞悬液稀释液中的病毒进行扩增，并将细胞培养的感染性终点与PCR以及另一种直接检测方法酶联免疫吸附测定（ELISA）进行比较。当相应的半数组织培养感染剂量（TCID50）终点分别为10^(-5.9)和10^(-5.7)时，PCR能够检测到10^(-4)稀释度的HSV I和HSV II病毒。ELISA仅能检测到10^(-1)稀释度的病毒。当初始蛋白酶消化后进行过滤时，扩增程序显示出最高的灵敏度。在需要快速结果的临床情况下，PCR可能可用于检测HSV。
