Abrahamsen L H, Jerkofsky M
Appl Environ Microbiol. 1983 May;45(5):1560-5. doi: 10.1128/aem.45.5.1560-1565.1983.
Further characterization of the viral enhancement system of varicella-zoster virus by the pesticide carbaryl (1-naphthyl-N-methylcarbamate) is presented. It was necessary to expose cells to the enhancing chemical during the period of virus replication to detect enhancement. The optimum time for the pretreatment is 20 to 24 h. Maximum enhancement of virus expression occurs 48 to 72 h post-inoculation. Treated cells cannot pass on to daughter cells the ability to produce increased amounts of virus. alpha-Naphthol, a metabolite of carbaryl, is also capable of enhancing virus replication; Guthion, another pesticide tested, did not enhance varicella-zoster virus. The stable cell line HEP-2 can be used in place of human embryonic lung cells to detect enhancement. Differences in enhancement levels were not due to cell lot, cell passage, or a change in the stability of the cell membrane to sonic disruption.
本文介绍了用杀虫剂西维因（1-萘基-N-甲基氨基甲酸酯）对水痘带状疱疹病毒的病毒增强系统进行的进一步特性研究。为了检测增强作用，有必要在病毒复制期间将细胞暴露于增强化学物质中。预处理的最佳时间为20至24小时。接种后48至72小时病毒表达出现最大增强。经处理的细胞不能将产生更多病毒的能力传递给子细胞。西维因的代谢产物α-萘酚也能够增强病毒复制；所测试的另一种杀虫剂谷硫磷不能增强水痘带状疱疹病毒。稳定细胞系HEP-2可用于替代人胚肺细胞来检测增强作用。增强水平的差异并非由于细胞批次、细胞传代或细胞膜对声波破坏稳定性的变化所致。
