Pape G R, Rieber E P, Eisenburg J, Hoffmann R, Balch C M, Paumgartner G, Riethmüller G
Gastroenterology. 1983 Sep;85(3):657-62.
So far, phenotypic and functional analyses of cytotoxic lymphocytes in viral hepatitis, as well as in primary biliary cirrhosis, have focused on circulating lymphocyte subpopulations, whereas their occurrence and distribution at the involved site, namely the liver, remain largely unknown. In the present study, monoclonal antibodies were used to characterize both circulating and liver-tissue-infiltrating lymphocyte subsets in acute cytomegalovirus hepatitis, in chronic B-virus hepatitis, and in primary biliary cirrhosis. Special emphasis was laid on the cytotoxic/suppressor T-cell subset. Total T cells were identified by the monoclonal antibody T411. The monoclonal antibody T811 was used to identify the cytotoxic/suppressor T-cell subset, which comprises virus-specific, altered self, and alloreactive cytolytic T lymphocytes and their precursors, a fraction of killer and natural killer cells. Furthermore, killer and natural killer cells were identified more specifically by the monoclonal antibody. HNK1. Irrespective of the number of cytotoxic/suppressor T cells in peripheral blood, these cells (T811 phenotype) were accumulated in the liver at the site of tissue injury. The preponderance of this lymphocyte subset at the site of tissue injury suggests an important role for these cells in the mechanism leading to tissue injury.
迄今为止，对于病毒性肝炎以及原发性胆汁性肝硬化中细胞毒性淋巴细胞的表型和功能分析，主要集中在循环淋巴细胞亚群上，而它们在受累部位即肝脏中的出现情况和分布状况，在很大程度上仍不清楚。在本研究中，使用单克隆抗体对急性巨细胞病毒性肝炎、慢性B病毒肝炎以及原发性胆汁性肝硬化中循环和肝组织浸润淋巴细胞亚群进行了表征。特别强调了细胞毒性/抑制性T细胞亚群。用单克隆抗体T411鉴定总T细胞。用单克隆抗体T811鉴定细胞毒性/抑制性T细胞亚群，该亚群包括病毒特异性、改变的自身以及同种异体反应性细胞溶解T淋巴细胞及其前体，一部分杀伤细胞和自然杀伤细胞。此外，用单克隆抗体HNK1更特异性地鉴定杀伤细胞和自然杀伤细胞。不管外周血中细胞毒性/抑制性T细胞的数量如何，这些细胞（T811表型）都在肝组织损伤部位积聚。该淋巴细胞亚群在组织损伤部位占优势，表明这些细胞在导致组织损伤的机制中起重要作用。
