Hansson B G, Calhoun J K, Wong D C, Feinstone S M, Purcell R H, Pannuti C S, Pereira J L, Koff R S, Dienstag J L, Iwarson S
Scand J Infect Dis. 1981;13(1):5-9. doi: 10.1080/00365548.1981.11690359.
A solid-phase radioimmunoassay for detecting specific IgM antibodies to hepatitis A virus (HAV) was developed and characterized. The test utilized microtiter plates coated with anti-IgM to specifically absorb the IgM antibodies from the test serum. The anti-hepatitis A IgM antibodies are measured by the specific consecutive binding of hepatitis A antigen and radiolabelled anti-hepatitis A antibodies (anti-HA). In 6 chimpanzees infected with HAV, IgM anti-HA was detected from about the first date of elevated transaminases and was positive for about 3 months. The usefulness of the test was confirmed by testing acute phase sera of 30 patients from a common source outbreak of epidemic hepatitis, and negative sera from 2 control groups. A collection of serum specimens from 190 patients with sporadic HBsAg-negative hepatitis in Brazil was also tested and an etiologic association with HAV was confirmed in the majority of these cases.
开发并鉴定了一种用于检测甲型肝炎病毒（HAV）特异性IgM抗体的固相放射免疫测定法。该检测采用包被抗IgM的微量滴定板，从检测血清中特异性吸附IgM抗体。甲型肝炎IgM抗体通过甲型肝炎抗原与放射性标记的抗甲型肝炎抗体（抗HA）的特异性连续结合来测定。在6只感染HAV的黑猩猩中，从转氨酶升高的大约第一天开始检测到IgM抗HA，并且在大约3个月内呈阳性。通过检测来自一次甲型肝炎共同来源暴发的30例患者的急性期血清以及2个对照组的阴性血清，证实了该检测方法的有效性。还对来自巴西190例散发性HBsAg阴性肝炎患者的血清标本进行了检测，在大多数病例中证实了与HAV的病因学关联。
