Casal M, Linares M J
Mycopathologia. 1983 Mar 22;81(3):155-9. doi: 10.1007/BF00436820.
The enzymatic profiles of several yeastlike organisms were studied using 19 substrates included in the API ZYM system. The isolates evaluated were: 186 Candida albicans, 19 C. stellatoidea, 4 C. tropicalis, 2 C. parapsilosis, 2 C. pseudotropicalis, 1 C. guilliermondii, 3 C. krusei, 11 Torulopsis (Candida) glabrata, 1 Cryptococcus neoformans, 2 Saccharomyces carlsbergensis, 1 Rhodotorula rubra, and 1 R. mucilaginosa. Esterase lipase (C8), leucine arylamidase, acid phosphatase, and phosphoamidase were detected in all of the isolates while trypsin and alpha-galactosidase were not found in any of the isolates using this system. The other enzymes were produced to a variable degree. The different enzymatic profiles might prove useful in the rapid differential diagnosis of genera and species of these yeastlike organisms. To this end, more extensive studies using more isolates of each species will be required, and enzymatic activity should be verified with other techniques and substrates.
使用API ZYM系统中包含的19种底物研究了几种类酵母微生物的酶谱。评估的分离株包括：186株白色念珠菌、19株星状念珠菌、4株热带念珠菌、2株近平滑念珠菌、2株假热带念珠菌、1株季也蒙念珠菌、3株克鲁斯念珠菌、11株光滑假丝酵母（念珠菌属）、1株新型隐球菌、2株卡尔斯伯酵母、1株深红酵母和1株粘红酵母。所有分离株均检测到酯酶脂肪酶（C8）、亮氨酸芳基酰胺酶、酸性磷酸酶和磷酸酰胺酶，而使用该系统在任何分离株中均未发现胰蛋白酶和α-半乳糖苷酶。其他酶的产生程度各不相同。不同的酶谱可能有助于这些类酵母微生物属和种的快速鉴别诊断。为此，需要使用更多每个物种的分离株进行更广泛的研究，并且酶活性应该用其他技术和底物进行验证。
