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RsrII——一种具有七核苷酸识别位点的新型限制性内切酶。

RsrII--a novel restriction endonuclease with a heptanucleotide recognition site.

作者信息

O'Connor C D, Metcalf E, Wrighton C J, Harris T J, Saunders J R

出版信息

Nucleic Acids Res. 1984 Sep 11;12(17):6701-8. doi: 10.1093/nar/12.17.6701.

DOI:10.1093/nar/12.17.6701
PMID:6091034
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC320110/
Abstract

A sequence-specific endonuclease present in extracts of Rhodopseudomonas sphaeroides 630 has been purified and characterized. The enzyme, Rsr II, recognises and cleaves the palindromic heptanucleotide sequence: (sequence; see test) By virtue of its unusual specificity, RsrII cuts most DNA molecules very infrequently which should facilitate the physical mapping of large genomes.

摘要

球形红假单胞菌630提取物中存在的一种序列特异性内切核酸酶已被纯化和表征。这种酶,即Rsr II,识别并切割回文七核苷酸序列:(序列;见测试)由于其独特的特异性,RsrII很少切割大多数DNA分子,这应该有助于大基因组的物理图谱绘制。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/468d/320110/36e4206fca33/nar00335-0068-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/468d/320110/6d75208d3ed0/nar00335-0066-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/468d/320110/44d3907df016/nar00335-0067-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/468d/320110/36e4206fca33/nar00335-0068-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/468d/320110/6d75208d3ed0/nar00335-0066-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/468d/320110/44d3907df016/nar00335-0067-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/468d/320110/36e4206fca33/nar00335-0068-a.jpg

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