RsrII——一种具有七核苷酸识别位点的新型限制性内切酶。
RsrII--a novel restriction endonuclease with a heptanucleotide recognition site.
作者信息
O'Connor C D, Metcalf E, Wrighton C J, Harris T J, Saunders J R
出版信息
Nucleic Acids Res. 1984 Sep 11;12(17):6701-8. doi: 10.1093/nar/12.17.6701.
Abstract
A sequence-specific endonuclease present in extracts of Rhodopseudomonas sphaeroides 630 has been purified and characterized. The enzyme, Rsr II, recognises and cleaves the palindromic heptanucleotide sequence: (sequence; see test) By virtue of its unusual specificity, RsrII cuts most DNA molecules very infrequently which should facilitate the physical mapping of large genomes.
摘要
球形红假单胞菌630提取物中存在的一种序列特异性内切核酸酶已被纯化和表征。这种酶，即Rsr II，识别并切割回文七核苷酸序列：（序列；见测试）由于其独特的特异性，RsrII很少切割大多数DNA分子，这应该有助于大基因组的物理图谱绘制。
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