Schols D, Verhaert P, Huybrechts R, Vaudry H, Jégou S, De Loof A
Histochemistry. 1987;86(4):345-51. doi: 10.1007/BF00494991.
Using the peroxidase-antiperoxidase technique, we showed the presence of peptides which are immunologically resembling mammalian corticotropin releasing hormone (CRF)-, adrenocorticotropic hormone (ACTH)-, beta-endorphin (beta-END)-, alpha-melanocyte stimulating hormone (alpha-MSH)-, methionine-enkephalin (met-ENK)- and leucine enkephalin (leu-ENK)- like immunoreactivity in hundreds to thousands of endocrine cells and nerve fibers in the midgut of the American cockroach Periplaneta americana. In the cockroach hindgut no immunoreactive cell bodies could be observed, although nerve fibers were clearly noticed to be recognized by antisera to CRF, ACTH1-24, ACTH11-24 and beta-END. Nothing is exactly known as to the function(s) of the demonstrated materials, but one can speculate that these numerous immunoreactive cells, might have important paracrine and/or endocrine functions in the insect physiology.
运用过氧化物酶-抗过氧化物酶技术，我们发现，在美洲大蠊（Periplaneta americana）中肠的数百至数千个内分泌细胞和神经纤维中，存在着与哺乳动物促肾上腺皮质激素释放激素（CRF）、促肾上腺皮质激素（ACTH）、β-内啡肽（β-END）、α-黑素细胞刺激素（α-MSH）、甲硫氨酸脑啡肽（met-ENK）及亮氨酸脑啡肽（leu-ENK）具有免疫相似性的肽类物质。在美国蟑螂后肠中，虽然能清楚地观察到神经纤维可被抗CRF、ACTH1-24、ACTH11-24及β-END的抗血清识别，但未观察到免疫反应性细胞体。对于所证实物质的功能，目前尚无确切了解，但可以推测，这些众多的免疫反应性细胞可能在昆虫生理学中具有重要的旁分泌和/或内分泌功能。
