Okii M, Iyobe S, Mitsuhashi S
J Bacteriol. 1983 Aug;155(2):643-9. doi: 10.1128/jb.155.2.643-649.1983.
We examined the aminoglycoside inactivation enzymes in Pseudomonas aeruginosa strains, seven clinical isolates and seven laboratory strains without plasmids. All strains were found to possess the enzyme aminoglycoside 3'-phosphotransferase II [APH(3')-II]. We isolated an APH(3')-II-deficient mutant from a PAO strain by mutagenesis with N-methyl-N'-nitro-N-nitrosoguanidine. By plasmid (FP5 or R68.45)-mediated conjugation, we determined the locus of the gene specifying the APH(3')-II between trp-6 and pro-82 on the PAO chromosome and designated this gene aphA. It was concluded that the intrinsic resistance of P. aeruginosa to kanamycins, neomycins, paromomycins, ribostamycin, and butirosins was due to this newly determined gene.
我们检测了铜绿假单胞菌菌株中的氨基糖苷失活酶，包括7株临床分离株和7株无质粒的实验室菌株。所有菌株均被发现含有氨基糖苷3'-磷酸转移酶II [APH(3')-II]。我们通过用N-甲基-N'-硝基-N-亚硝基胍诱变，从PAO菌株中分离出一株APH(3')-II缺陷型突变体。通过质粒（FP5或R68.45）介导的接合，我们确定了PAO染色体上trp-6和pro-82之间指定APH(3')-II的基因位点，并将该基因命名为aphA。得出的结论是，铜绿假单胞菌对卡那霉素、新霉素、巴龙霉素、核糖霉素和丁胺卡那霉素的固有抗性归因于这个新确定的基因。
