Makino S, Taguchi F, Hayami M, Fujiwara K
Microbiol Immunol. 1983;27(5):445-54. doi: 10.1111/j.1348-0421.1983.tb00603.x.
Two small plaque mutants designated as 1a and 2c were isolated from DBT cells persistently infected with the JHM strain of mouse hepatitis virus. Unlike the wild type JHM, these two mutant viruses grew more slowly with no prominent cell fusion. The buoyant densities of the mutants were slightly lower and 2c was revealed to have fewer peplomers than JHM by electron microscopy. The purified JHM contained five polypeptides with molecular weights (M.W.) of 260,000, 105,000 (GP105), 65,000, 60,000 (P60), and 23,000 (GP23). In addition to two polypeptides, P60 and GP23, which were common to JHM and the mutants, 1a was found to contain three other specific polypeptides with M.W. of 180,000 (GP180), 110,000, and 95,000 (GP95), while 2c had GP180, GP105, GP95, and one with a M.W. of 175,000. All of these polypeptides were shown to be glycosylated except for P60. After bromelain treatment, all these viruses lost the peplomers and contained P60 and another new 18,000 dalton polypeptide.
从持续感染小鼠肝炎病毒JHM株的DBT细胞中分离出两个小斑块突变体，分别命名为1a和2c。与野生型JHM不同，这两种突变病毒生长较慢，没有明显的细胞融合现象。突变体的浮力密度略低，通过电子显微镜观察发现2c的纤突比JHM少。纯化的JHM含有五种分子量分别为260,000、105,000（GP105）、65,000、60,000（P60）和23,000（GP23）的多肽。除了JHM和突变体共有的两种多肽P60和GP23外，发现1a还含有另外三种分子量分别为180,000（GP180）、110,000和95,000（GP95）的特异性多肽，而2c含有GP180、GP105、GP95和一种分子量为175,000的多肽。除P60外，所有这些多肽都被证明是糖基化的。用菠萝蛋白酶处理后，所有这些病毒都失去了纤突，并含有P60和另一种新的18,000道尔顿的多肽。
