Humphries E H, Danhof M L, Hlozanek I
Virology. 1984 May;135(1):125-38. doi: 10.1016/0042-6822(84)90123-5.
Five lines of chickens have been examined for the presence of DNA sequences related to the endogenous avian retrovirus. Five new loci have been identified, based upon analysis with the restriction endonucleases SacI and BamHI. One locus has been associated with the production of infectious endogenous virus. Restriction endonuclease mapping suggested a limited similarity between the flanking cellular sequences of two of these loci, ev-17 and ev-18, and several endogenous loci, including ev-1, already characterized. The data suggested that these two loci might have been generated by chromosomal duplication. Hybridization analysis with a probe containing the cellular sequences that flank ev-1, however, revealed that these flanking sequences shared no detectable homology with the cellular sequences that surround ev-17, ev-18, or nine other endogenous loci that were examined. These results are consistent with the hypothesis that several of the endogenous viral loci resulted either from independent infections of the germ line or from virus transpositions.
已对五个品系的鸡进行检测，以确定是否存在与内源性禽逆转录病毒相关的DNA序列。基于用限制性内切酶SacI和BamHI进行的分析，已鉴定出五个新位点。其中一个位点与传染性内源性病毒的产生有关。限制性内切酶图谱分析表明，其中两个位点ev-17和ev-18的侧翼细胞序列与几个已鉴定的内源性位点（包括ev-1）之间存在有限的相似性。数据表明，这两个位点可能是由染色体复制产生的。然而，用包含ev-1侧翼细胞序列的探针进行杂交分析发现，这些侧翼序列与围绕ev-17、ev-18或其他九个检测的内源性位点的细胞序列没有可检测到的同源性。这些结果与以下假设一致，即几个内源性病毒位点要么是由生殖系的独立感染产生，要么是由病毒转座产生。
