嗜碱芽孢杆菌属的青霉素酶通过大肠杆菌外膜的排泄。
Excretion of the penicillinase of an alkalophilic Bacillus sp. through the Escherichia coli outer membrane.
作者信息
Kudo T, Kato C, Horikoshi K
出版信息
J Bacteriol. 1983 Nov;156(2):949-51. doi: 10.1128/jb.156.2.949-951.1983.
Abstract
Two plasmids containing the penicillinase gene of alkalophilic Bacillus sp. strain 170, pEAP1 and pEAP2, were constructed. Most of the penicillinase produced by Escherichia coli, which carried these plasmids, was found in the culture medium. This excretion is caused by the cloned DNA fragment which contains some component that changes the outer membrane of E. coli.
摘要
构建了两个含有嗜碱芽孢杆菌170株青霉素酶基因的质粒，即pEAP1和pEAP2。携带这些质粒的大肠杆菌产生的大部分青霉素酶存在于培养基中。这种分泌是由克隆的DNA片段引起的，该片段包含一些能改变大肠杆菌外膜的成分。
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