Maarse A C, Van Loon A P, Riezman H, Gregor I, Schatz G, Grivell L A
EMBO J. 1984 Dec 1;3(12):2831-7. doi: 10.1002/j.1460-2075.1984.tb02216.x.
The six small subunits (IV-VII, VIIa, VIII) of yeast cytochrome c oxidase are encoded by nuclear genes and imported into the mitochondria. We have isolated the gene for subunit IV from a yeast genomic clone bank and determined its complete nucleotide sequence. We have also isolated subunit IV from purified yeast cytochrome c oxidase and determined most of its amino acid sequence which confirms the positioning of approximately 90% of the amino acid residues. The sequence comparison shows that the coding sequence of the gene lacks introns and that subunit IV is made as a precursor with an amino-terminal extension of 25 residues, five of which are basic and none of them acidic. Precursor processing involves cleavage of a Leu-Gln bond.
酵母细胞色素c氧化酶的六个小亚基（IV-VII、VIIa、VIII）由核基因编码并导入线粒体。我们从酵母基因组克隆文库中分离出亚基IV的基因，并确定了其完整的核苷酸序列。我们还从纯化的酵母细胞色素c氧化酶中分离出亚基IV，并确定了其大部分氨基酸序列，这证实了约90%的氨基酸残基的定位。序列比较表明，该基因的编码序列没有内含子，亚基IV作为前体合成，其氨基末端有25个残基的延伸，其中五个是碱性的，没有一个是酸性的。前体加工涉及Leu-Gln键的切割。
