Symons R H
Nucleic Acids Res. 1981 Dec 11;9(23):6527-37. doi: 10.1093/nar/9.23.6527.
The sequence of the 247 nucleotide residues of the single strand circular RNA of avocado sunblotch viroid (ASBV) was determined using partial enzymic cleavage methods on overlapping viroid fragments obtained by partial ribonuclease digestion followed by 32p-labelling in vitro at their 5'-ends. ASBV is much smaller than potato spindle tuber viroid (PSTV; 359 residues) and chrysanthemum stunt viroid (CSV; 356 residues). A secondary structure model for ASBV is proposed and contains 67% of its residues base paired. In contrast to the extensive (69%) sequence homology of CSV with PSTV, only 18% of the ASBV sequence is homologous to PSTV and CSV. There are eight potential polypeptide translation products with chain lengths from 4 to 63 amino acid residues coded for by the plus (infectious) strand and four potential translation products (2 to 60 residues) coded for by the minus strand. An improved method is described for the synthesis of gamma-32p-ATP of high specific activity.
利用部分酶切方法，对鳄梨日斑类病毒（ASBV）单链环状RNA的247个核苷酸残基序列进行了测定。这些片段是通过部分核糖核酸酶消化获得的重叠类病毒片段，然后在体外对其5'端进行³²P标记。ASBV比马铃薯纺锤块茎类病毒（PSTV；359个残基）和菊花矮化类病毒（CSV；356个残基）小得多。本文提出了ASBV的二级结构模型，其67%的残基形成碱基对。与CSV和PSTV广泛的（69%）序列同源性相反，ASBV序列中只有18%与PSTV和CSV同源。正链（感染性链）编码8种潜在的多肽翻译产物，链长从4到63个氨基酸残基，负链编码4种潜在的翻译产物（2到60个残基）。本文描述了一种合成高比活γ-³²P-ATP的改进方法。
