Lambert G, Carr N
Plasmid. 1983 Sep;10(2):196-8. doi: 10.1016/0147-619x(83)90072-0.
A plasmid designated pDC1 from the cyanobacterium Nostoc sp. MAC PCC 8009 was incubated with 16 different restriction enzymes, of which 8 cleaved pDC1. Plasmid pDC1 has a single site for ClaI, two sites for each of BglI, EcoRI, EcoRV, and MluI, three sites for HpaI, and four for HindIII. A restriction map of pDC1 for these 7 enzymes was constructed.
将来自蓝藻念珠藻属MAC PCC 8009的名为pDC1的质粒与16种不同的限制酶一起孵育,其中8种能切割pDC1。质粒pDC1有一个ClaI位点、BglI、EcoRI、EcoRV和MluI各有两个位点、HpaI有三个位点、HindIII有四个位点。构建了这7种酶的pDC1限制酶图谱。