作为IS2插入热点靶点的噬菌体P1基因组区域的序列。
The sequence of the bacteriophage P1 genome region serving as hot target for IS2 insertion.
作者信息
Sengstag C, Shepherd J C, Arber W
出版信息
EMBO J. 1983;2(10):1777-81. doi: 10.1002/j.1460-2075.1983.tb01657.x.
Abstract
A restriction fragment of the bacteriophage P1 genome known to serve as a hot target for IS2 insertion in its host, Escherichia coli K12, was entirely sequenced. It is 1756 bp long and it contains four long open reading frames, all in the same orientation. The two middle frames overlap partially. Eight of the nine studied IS2 insertions affecting phage reproduction map within three of these reading frames. No common feature was found between the nine target sites which have served for IS2 integration. However, there are two structural elements which might possibly contribute to rendering the studied DNA segment a hot region for IS2 insertion. The first is formed by two neighbouring, 30 and 40 bp regions of homology with an internal segment of IS2. The second is the pentanucleotide 5' GGTAT3', which is carried nine times in the sequenced fragment and which is found always in at least one copy within a variable distance of less than 100 bp of each inserted IS2 element.
摘要
对噬菌体P1基因组的一个限制性片段进行了全序列测定，该片段在其宿主大肠杆菌K12中是IS2插入的热点。它长1756 bp，包含四个长的开放阅读框，方向均相同。中间的两个阅读框部分重叠。影响噬菌体繁殖的九个研究的IS2插入中有八个位于其中三个阅读框内。在用于IS2整合的九个靶位点之间未发现共同特征。然而，有两个结构元件可能有助于使所研究的DNA片段成为IS2插入的热点区域。第一个元件由与IS2内部片段相邻的两个同源区域（30和40 bp）组成。第二个元件是五核苷酸5'GGTAT3'，在测序片段中出现了九次，并且在每个插入的IS2元件的小于100 bp的可变距离内总是至少有一个拷贝。
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本文引用的文献
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