人类基因组中 HindIII 1.8-kb 重复序列家族的二维凝胶电泳分析。

Two-dimensional gel electrophoretic analysis of the HindIII 1.8-kb repetitive-sequence family in the human genome.

作者信息

Sakaki Y, Kurata Y, Miyake T, Saigo K

出版信息

Gene. 1983 Oct;24(2-3):179-90. doi: 10.1016/0378-1119(83)90078-1.

Abstract

The structure and organization of a human repetitive DNA family containing the HindIII 1.8-kb repetitive sequence were studied, using two-dimensional (2D) gel electrophoresis. The HindIII 1.8-kb sequence proved to be part of a repetitive sequence about 5 kb long and interspersed on the genome. The long repetitive sequence family contained several subgroups, as based on polymorphism of the restriction site. Recombinant phages containing the long repetitive sequence were isolated from the human genomic DNA library. Heteroduplex and restriction analysis showed that the structure of the repetitive sequence carried by the phages was close to that expected from 2D gel electrophoretic analysis. The 2D gel electrophoretic analysis was shown to be a reliable and useful approach for surveying and mass analysis of repetitive sequence families.

摘要

利用二维（2D）凝胶电泳技术，对包含HindIII 1.8kb重复序列的人类重复DNA家族的结构和组织进行了研究。结果证明，HindIII 1.8kb序列是一个约5kb长的重复序列的一部分，且散布于基因组中。基于限制性酶切位点的多态性，该长重复序列家族包含几个亚组。从人类基因组DNA文库中分离出了含有该长重复序列的重组噬菌体。异源双链体和限制性分析表明，噬菌体携带的重复序列结构与二维凝胶电泳分析预期的结构相近。二维凝胶电泳分析被证明是一种用于重复序列家族调查和大量分析的可靠且有用的方法。