一种主要的人类重复DNA（Hind III 1.9 kb家族）的核苷酸序列定义
Nucleotide sequence definition of a major human repeated DNA, the Hind III 1.9 kb family.
作者信息
Manuelidis L
出版信息
Nucleic Acids Res. 1982 May 25;10(10):3211-9. doi: 10.1093/nar/10.10.3211.
Abstract
A human Hind III 1.9 kb repeated DNA fragment was isolated and cloned in pBR322. A cloned member that hybridized predominantly to the 1.9 kb Hind III band in a digest of whole human DNA was chosen for sequencing. It is an 1894 bp fragment that shows no significant internal repeats. Few pCG residues are observed in the sequence and there are numerous stop codons. Detailed sequence comparisons confirm this is a novel class of repeats that is not related to previously characterized human satellite DNAs or Alu sequences. At least a portion of the sequence described is conserved in evolution.
摘要
分离出一个人类Hind III 1.9 kb重复DNA片段，并克隆到pBR322中。从全人类DNA消化产物中选择了一个主要与1.9 kb Hind III条带杂交的克隆成员进行测序。它是一个1894 bp的片段，没有明显的内部重复序列。在该序列中观察到很少的pCG残基，并且有许多终止密码子。详细的序列比较证实这是一类新的重复序列，与先前鉴定的人类卫星DNA或Alu序列无关。所述序列的至少一部分在进化中是保守的。
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