Schoner B, Kelly S, Smith H O
Gene. 1983 Oct;24(2-3):227-36. doi: 10.1016/0378-1119(83)90083-5.
We have determined the nucleotide sequence of a cloned 1710-bp segment of Haemophilus haemolyticus DNA which contains the HhaII restriction (r) and modification (m) genes. The m gene is 513 bp in length and the rgene is 681 bp in length. Both are in the same reading frame, being separated by a 21-bp region. A ribosome-binding site is identified in front of each gene, but no Haemophilus promoter is apparent on the cloned fragment. Transcription originates from a plasmid promotor and proceeds in the direction m to r.
我们已经确定了溶血嗜血杆菌DNA克隆的1710碱基对片段的核苷酸序列，该片段包含HhaII限制（r）和修饰（m）基因。m基因长度为513碱基对，r基因长度为681碱基对。两者处于同一阅读框，由一个21碱基对的区域隔开。在每个基因前面都鉴定出一个核糖体结合位点，但在克隆片段上没有明显的嗜血杆菌启动子。转录从质粒启动子开始，按m到r的方向进行。
