Naundorf H, Fichtner I, Elbe B, Saul G J, Haensch W, Zschiesche W, Reinecke S
Max-Delbrück-Centrum for Molecular Medicine, Berlin-Buch, Germany.
Breast Cancer Res Treat. 1994;32(2):187-96. doi: 10.1007/BF00665769.
Two new human mammary carcinoma lines originating from surgical material were established in nude mice. According to the adopted criteria, the tumor 4049 has been classified as estradiol receptor positive and mammary carcinoma 4296 as estradiol receptor negative. Both tumors proved to be c-erbB-2 protein positive and EGF-receptor negative. In contrast to carcinoma 4296, the in vitro growth and the take rate of mammary carcinoma 4049 in nude mice seems to be dependent on stromal components. Pretreatment of mice with estradiol/peanut oil before tumor engraftment was an essential precondition for the growth of the primary tumor in nude mice. After successful establishment the tumor growth was significantly stimulated by estradiol. The growth rate of mammary carcinoma 4296 was independent of any supplementation of estradiol. The two breast tumors were characterized with regard to their growth behaviour, histology, and sensitivity to cytostatics and antihormones. They are considered suitable tumor models for the testing of antineoplastic substances and for biological experiments.
从手术材料中获取的两种新的人类乳腺癌细胞系在裸鼠体内建立。根据所采用的标准，肿瘤4049被归类为雌激素受体阳性，而乳腺癌4296为雌激素受体阴性。两种肿瘤均被证明c-erbB-2蛋白呈阳性，表皮生长因子受体呈阴性。与癌4296不同，乳腺癌4049在裸鼠体内的体外生长和接种率似乎依赖于基质成分。在肿瘤植入前用雌二醇/花生油对小鼠进行预处理是裸鼠体内原发性肿瘤生长的必要前提条件。成功建立后，雌二醇可显著刺激肿瘤生长。乳腺癌4296的生长速率与雌二醇的任何补充无关。对这两种乳腺肿瘤在生长行为、组织学以及对细胞抑制剂和抗激素的敏感性方面进行了表征。它们被认为是用于测试抗肿瘤物质和进行生物学实验的合适肿瘤模型。
