de Graaf J S, de Vos C J, Steenbergen H J
J Pharmacol Methods. 1983 Sep;10(2):113-35. doi: 10.1016/0160-5402(83)90074-8.
A fully automated system for pharmacological in vitro experiments with isolated tissues is described. The apparatus consists of an organ bath equipped with a) a gradient pump supplying a logarithmic concentration/time gradient of agonist; b) pumps and valves for dispensing bath fluid, antagonist solutions, and an oxygenation gas mixture; and c) a transducer with automatic baseline adjustment. The information coming from the preparation (i.e., changes in length or tension) is fed into a DEC PDP-11 mini-computer (MINC). This computer controls the test procedure either directly (e.g., when to break off the logarithmic gradient) or indirectly after processing the information in the form of log-concentration response curves (e.g., when to repeat a given procedure, or when to stop the experiment). At the end of the experiment, the results are presented both alpha-numerically and graphically. The data of various experiments can be accumulated and Schild-plots obtained. The example given demonstrates that this automated method offers considerable advantages over the classical manual method in that it has far greater precision. By careful planning, using several set-ups simultaneously with different isolated organs from the same animal, the investigator can carry out various experiments 24 hr a day and also reduce the number of sacrificed animals.
本文描述了一种用于离体组织药理体外实验的全自动系统。该仪器由一个器官浴槽组成，其配备有：a) 一个梯度泵，用于提供激动剂的对数浓度/时间梯度；b) 用于分配浴液、拮抗剂溶液和氧化气体混合物的泵和阀门；以及c) 一个具有自动基线调整功能的换能器。来自标本（即长度或张力的变化）的信息被输入到一台DEC PDP - 11小型计算机（MINC）中。这台计算机直接控制测试过程（例如，何时中断对数梯度），或者在以对数浓度响应曲线的形式处理信息后间接控制（例如，何时重复给定程序，或何时停止实验）。在实验结束时，结果以字母数字和图形形式呈现。可以累积各种实验的数据并获得施尔德图。所举的例子表明，这种自动化方法比传统的手动方法具有相当大的优势，因为它具有更高的精度。通过精心规划，同时使用来自同一只动物的不同离体器官的多个装置，研究人员可以一天24小时进行各种实验，并且还能减少牺牲动物的数量。
