Meyers-Elliott R H, Chitjian P A, Dethlefs B A
Ophthalmic Res. 1983;15(5):240-56. doi: 10.1159/000265266.
Intrastromal and topical routes of infection of rabbit corneas with the HF strain of herpes simplex virus type 1 were compared clinically to determine which route of infection would present the best model of disciform edema and of deep stromal keratitis for our further studies on how the immune response of the infected animals may influence the expression of clinical disease. In addition, virus clearing and persistence of viral antigens as immunologic stimuli were monitored by virologic and electron microscopic immunocytochemical studies. We found topical infection to be preferable to the intrastromal infection route for presenting stromal disease in that it resulted in a higher incidence of epithelial disease with less anterior chamber involvement. The topical infection route model should be valuable in studies of cell-mediated immune response against HSV-infected cells.
对兔角膜采用1型单纯疱疹病毒HF株进行基质内和局部感染途径的临床比较，以确定哪种感染途径能为我们进一步研究受感染动物的免疫反应如何影响临床疾病的表现提供最佳的盘状水肿和深层基质角膜炎模型。此外，通过病毒学和电子显微镜免疫细胞化学研究监测病毒清除情况以及作为免疫刺激物的病毒抗原的持续存在情况。我们发现，就引发基质疾病而言，局部感染比基质内感染途径更可取，因为它导致上皮疾病的发生率更高，前房受累更少。局部感染途径模型在针对单纯疱疹病毒感染细胞的细胞介导免疫反应研究中应具有重要价值。
