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一段重复的大鼠DNA片段的核苷酸序列。

Nucleotide sequence of a reiterated rat DNA fragment.

作者信息

Gupta R C

出版信息

FEBS Lett. 1983 Nov 28;164(1):175-80. doi: 10.1016/0014-5793(83)80044-1.

DOI:10.1016/0014-5793(83)80044-1
PMID:6317441
Abstract

A reiterated component of rat DNA was isolated by restriction with HindIII endonuclease and polyacrylamide gel electrophoresis. Sequence analysis revealed that the fragment was 179 nucleotides long. Unlike the known 370N reiterated rat DNA fragment which contained a high m5C content (2.7 mole%), this repetitive element contained a rather low m5C content (0.5 mole%). The present rat repetitive sequence appeared to be of alpha-type as shown by its significant homologies with alpha DNA sequences of African green monkey and human. The possibility of sequence heterogeneity is discussed.

摘要

通过用HindIII核酸内切酶进行酶切及聚丙烯酰胺凝胶电泳，分离出大鼠DNA的一个重复组分。序列分析表明，该片段长度为179个核苷酸。与已知的含有高m5C含量（2.7摩尔%）的370N大鼠重复DNA片段不同，这个重复元件的m5C含量相当低（0.5摩尔%）。如与非洲绿猴和人类的α DNA序列具有显著同源性所示，目前的大鼠重复序列似乎属于α型。文中讨论了序列异质性的可能性。

相似文献

1
Nucleotide sequence of a reiterated rat DNA fragment.一段重复的大鼠DNA片段的核苷酸序列。
FEBS Lett. 1983 Nov 28;164(1):175-80. doi: 10.1016/0014-5793(83)80044-1.
2
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Nucleotide sequence of a highly repetitive component of rat DNA.大鼠DNA高度重复组分的核苷酸序列。
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引用本文的文献

1
Nonrandom binding of the carcinogen N-hydroxy-2-acetylaminofluorene to repetitive sequences of rat liver DNA in vivo.致癌物N-羟基-2-乙酰氨基芴在体内与大鼠肝脏DNA重复序列的非随机结合。
Proc Natl Acad Sci U S A. 1984 Nov;81(22):6943-7. doi: 10.1073/pnas.81.22.6943.
2
Rat LINE1: the origin and evolution of a family of long interspersed middle repetitive DNA elements.大鼠长散在核元件1：一个长散在中等重复DNA元件家族的起源与进化
J Mol Evol. 1985;22(2):117-33. doi: 10.1007/BF02101690.