高度重复的非洲绿猴DNA单重复单元之间的序列关系。
Sequence relationships between single repeat units of highly reiterated African Green monkey DNA.
作者信息
Thayer R E, Singer M F, McCutchan T F
出版信息
Nucleic Acids Res. 1981 Jan 10;9(1):169-81. doi: 10.1093/nar/9.1.169.
Abstract
Individual monomer and dimer units of the highly repeated alpha-component DNA of African Green monkeys were isolated and amplified by molecular cloning in pBR322. The purified sequences were characterized by digestion with restriction endonucleases and by primary nucleotide sequence analysis. Comparison of the cloned units with the 172 base pair long sequence representing the most abundant nucleotide at each position in the set of sequences comprising alpha-component allows the following conclusions. The set of sequences comprising alpha-component is made up of a very large number of related but slightly divergent sequences. Two neighboring repeats of the monomer unit are not necessarily more similar to one another than are randomly isolated monomers.
摘要
通过在pBR322中进行分子克隆，分离并扩增了非洲绿猴高度重复的α-组分DNA的单个单体和二聚体单元。通过用限制性内切酶消化和一级核苷酸序列分析对纯化的序列进行了表征。将克隆的单元与代表α-组分序列集合中每个位置最丰富核苷酸的172个碱基对长的序列进行比较，得出以下结论。构成α-组分的序列集合由大量相关但略有差异的序列组成。单体单元的两个相邻重复序列彼此之间的相似性不一定比随机分离的单体更高。
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