Eisenberg R J, Long D, Hogue-Angeletti R, Cohen G H
J Virol. 1984 Jan;49(1):265-8. doi: 10.1128/JVI.49.1.265-268.1984.
Glycoprotein D (gD) of herpes simplex virus is a structural component of the virion envelope which stimulates production of high titers of herpes simplex virus type-common neutralizing antibody. We carried out automated N-terminal amino acid sequencing studies on radiolabeled preparations of gD-1 (gD of herpes simplex virus type 1) and gD-2 (gD of herpes simplex virus type 2). Although some differences were noted, particularly in the methionine and alanine profiles for gD-1 and gD-2, the amino acid sequence of a number of the first 30 residues of the amino terminus of gD-1 and gD-2 appears to be quite similar. For both proteins, the first residue is a lysine. When we compared our sequence data for gD-1 with those predicted by nucleic acid sequencing, the two sequences could be aligned (with one exception) starting at residue 26 (lysine) of the predicted sequence. Thus, the first 25 amino acids of the predicted sequence are absent from the polypeptides isolated from infected cells.
单纯疱疹病毒的糖蛋白D（gD）是病毒粒子包膜的一种结构成分，可刺激产生高滴度的单纯疱疹病毒通用中和抗体。我们对放射性标记的gD-1（单纯疱疹病毒1型的gD）和gD-2（单纯疱疹病毒2型的gD）制剂进行了自动N端氨基酸测序研究。尽管发现了一些差异，特别是gD-1和gD-2的甲硫氨酸和丙氨酸谱，但gD-1和gD-2氨基末端前30个残基中的一些氨基酸序列似乎非常相似。对于这两种蛋白质，第一个残基都是赖氨酸。当我们将gD-1的序列数据与核酸测序预测的序列进行比较时，从预测序列的第26个残基（赖氨酸）开始，这两个序列可以对齐（有一个例外）。因此，从感染细胞中分离的多肽中不存在预测序列的前25个氨基酸。
