Dean H J, Warner M S, Terhune S S, Johnson R M, Spear P G
Department of Microbiology-Immunology, Northwestern University Medical School, Chicago, Illinois 60611, USA.
J Virol. 1995 Aug;69(8):5171-6. doi: 10.1128/JVI.69.8.5171-5176.1995.
Cells that express glycoprotein D (gD) of herpes simplex virus type 1 (HSV-1) resist infection by HSV-1 and HSV-2 because of interference with viral penetration. The results presented here show that both HSV-1 and HSV-2 gD can mediate interference and that various HSV-1 and HSV-2 strains differ in sensitivity to this interference. The relative degree of sensitivity was not necessarily dependent on whether the cell expressed the heterologous or homologous form of gD but rather on the properties of the virus. Marker transfer experiments revealed that the allele of gD expressed by the virus was a major determinant of sensitivity to interference. Amino acid substitutions in the most distal part of the gD ectodomain had a major effect, but substitutions solely in the cytoplasmic domain also influenced sensitivity to interference. In addition, evidence was obtained that another viral gene(s) in addition to the one encoding gD can influence sensitivity to interference. The results indicate that HSV-1 and HSV-2 gD share determinants required to mediate interference with infection by HSV of either serotype and that the pathway of HSV entry that is blocked by expression of cell-associated gD can be cleared or bypassed through subtle alterations in virion-associated proteins, particularly gD.
表达单纯疱疹病毒1型（HSV-1）糖蛋白D（gD）的细胞可抵抗HSV-1和HSV-2的感染，原因是其对病毒穿透具有干扰作用。本文给出的结果表明，HSV-1和HSV-2的gD均可介导干扰作用，并且不同的HSV-1和HSV-2毒株对这种干扰的敏感性存在差异。敏感性的相对程度不一定取决于细胞表达的是gD的异源形式还是同源形式，而是取决于病毒的特性。标记转移实验表明，病毒表达的gD等位基因是干扰敏感性的主要决定因素。gD胞外域最远端部分的氨基酸替换具有主要影响，但仅在胞质域的替换也会影响对干扰的敏感性。此外，有证据表明，除了编码gD的基因外，另一个病毒基因也可影响对干扰的敏感性。结果表明，HSV-1和HSV-2的gD具有介导对任一血清型HSV感染干扰所需的共同决定因素，并且通过病毒体相关蛋白（特别是gD）的细微改变，可以清除或绕过因细胞相关gD表达而被阻断的HSV进入途径。
