Noguchi Norihisa, Ohashi Takashi, Shiratori Taisei, Narui Koji, Hagiwara Tadashi, Ko Mari, Watanabe Kiyoshi, Miyahara Takeo, Taira Satoru, Moriyasu Fuminori, Sasatsu Masanori
Department of Microbiology, School of Pharmacy, Tokyo University of Pharmacy and Life Science, 1432-1 Horinouchi, Hachioji, Tokyo 192-0392, Japan.
J Gastroenterol. 2007 May;42(5):346-51. doi: 10.1007/s00535-007-2012-5. Epub 2007 May 25.
The relationship between Streptococcus (St.) bovis endocarditis and colon cancer is well known. In St. bovis, the biotype I strain (formerly, St. gallolyticus) produces tannase that degrades tannins. The aim of this study was to investigate the association of tannase-producing bacteria with colon cancer, and to identify the major tannase-producing bacteria and the gene involved.
Tannase-producing bacteria were isolated in tannic acid-treated selective agar medium from feces and rectal swabs of 357 patients who underwent colon endoscopy from 1999 to 2004.
Tannase-producing bacteria were isolated more frequently from the colon cancer group (24.3%) than from the adenoma or normal groups (14.4%; P < 0.05). S. gallolyticus, Staphylococcus (S.) lugdunensis, Lactobacillus (L.) plantarum, and L. pentosus were all identified as tannase-producing bacteria. Of these, S. lugdunensis was significantly isolated from the advanced-stage cancer group (22.2%; P < 0.001) more than from the early-stage cancer (8.6%) or adenoma (4.9%) groups. The gene (tanA) for tannase in S. lugdunensis was cloned and sequenced. The tanA gene was associated with all S. lugdunensis but not with other bacteria by Southern blotting and polymerase chain reaction.
Tannase-producing S. lugdunensis is associated with advanced-stage colon cancer, and the tanA gene is a useful marker for the detection of S. lugdunensis.
牛链球菌心内膜炎与结肠癌之间的关系已为人熟知。在牛链球菌中，I型生物型菌株（以前称为解没食子酸链球菌）可产生降解单宁的单宁酶。本研究的目的是调查产单宁酶细菌与结肠癌的关联，并鉴定主要的产单宁酶细菌及相关基因。
1999年至2004年接受结肠镜检查的357例患者的粪便和直肠拭子，在经单宁酸处理的选择性琼脂培养基中分离出产单宁酶细菌。
产单宁酶细菌在结肠癌组中的分离频率（24.3%）高于腺瘤组或正常组（14.4%；P<0.05）。解没食子酸链球菌、路邓葡萄球菌、植物乳杆菌和戊糖乳杆菌均被鉴定为产单宁酶细菌。其中，路邓葡萄球菌在晚期癌症组中的分离率（22.2%；P<0.001）显著高于早期癌症组（8.6%）或腺瘤组（4.9%）。对路邓葡萄球菌中的单宁酶基因（tanA）进行了克隆和测序。通过Southern印迹法和聚合酶链反应，tanA基因与所有路邓葡萄球菌相关，而与其他细菌无关。
产单宁酶的路邓葡萄球菌与晚期结肠癌相关，tanA基因是检测路邓葡萄球菌的有用标志物。
