Wada H, Takahashi N, Yoshino H, Iwamoto T
Yakubutsu Seishin Kodo. 1983 Sep;3(2):55-65.
An intraseptal 6-hydroxydopamine (6-OHDA) injection technique was used in order to examine the specific functions of the catecholamine (CA) neurons in the septal area upon the time discrimination behavior. After some preliminary CRF trainings of 5 days, 21 rats of Wistar strain were divided into 3 homogeneous groups according to their performance on the last session. 6-OHDA group (n = 6) was stereotaxically injected into the medial septal area with 8 micrograms of 6-OHDA in 0.9% saline solution containing 0.1% ascorbic acid. Ascorbic acid group (n = 5) was injected with only ascorbic acid solution and the remaining group (n = 10) received no treatments. Following the 6 recovery days and the next CRF trainings of 5 days, all subjects were exposed to a DRLL 20/LH 36 training for 30 min per day and consecutively for 30 days. As the results of these trainings, 6-OHDA group showed significantly better performance and less acceleration of operant responding just after incorrect (non-rewarded) responses as compared with other control groups in the early 10 sessions of the trainings. These findings suggested that the acquisition of time discrimination behavior was facilitated in the 6-OHDA group which was hardly affected by the emotional disturbance just after non-rewards. Therefore the authors consider that the emotional disturbance caused by non-rewards impairs the inhibition of operant responding by mediating the CA neurons in the septal area.
为了研究隔区儿茶酚胺（CA）神经元在时间辨别行为中的特定功能，采用了隔区内注射6-羟基多巴胺（6-OHDA）的技术。在进行了为期5天的初步条件反射训练后，根据21只Wistar品系大鼠在最后一次训练中的表现，将它们分成3个同质组。6-OHDA组（n = 6）通过立体定位向内侧隔区注射含0.1%抗坏血酸的0.9%盐溶液中的8微克6-OHDA。抗坏血酸组（n = 5）仅注射抗坏血酸溶液，其余组（n = 10）不进行处理。在6天的恢复和接下来5天的条件反射训练后，所有实验对象每天接受30分钟的差异强化低率20/高率36训练，持续30天。作为这些训练的结果，在训练的前10节中，与其他对照组相比，6-OHDA组表现出明显更好的成绩，并且在错误（无奖励）反应后操作反应的加速更少。这些发现表明，6-OHDA组时间辨别行为的获得得到促进，且几乎不受无奖励后情绪干扰的影响。因此，作者认为无奖励引起的情绪干扰通过介导隔区的CA神经元损害了对操作反应的抑制。
