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对选定零售食品的微生物学及活菌计数方法的评估。

The microbiology of selected retail food products with an evaluation of viable counting methods.

作者信息

Greenwood M H, Coetzee E F, Ford B M, Gill P, Hooper W L, Matthews S C, Patrick S, Pether J V, Scott R J

出版信息

J Hyg (Lond). 1984 Feb;92(1):67-77. doi: 10.1017/s0022172400064044.

Abstract

In an inter-laboratory survey, the pour plate, surface spread, agar droplet and spiral plate methods were used in parallel with the surface drop method for enumeration of micro-organisms in foods. Good agreement was obtained between all surface methods of enumeration, but there was poor agreement between molten agar methods and the surface drop method. A total of 1143 samples of food that were ready for consumption at the point of retail sale were examined. Eight types of food products were chosen: meat pasties, sausage rolls, real-cream slices, synthetic-cream slices, mayonnaise-based coleslaws, faggots, patés and continental sausages. The results of this survey suggest that the upper limit for an acceptable viable count should vary according to the food product. Salmonellae were not isolated on any occasion. Potentially harmful organisms were not isolated at levels expected to constitute a public health hazard. Information concerning the nature of the product, the total viable count, the presence or absence of pathogenic, toxigenic or indicator organisms, the spectrum of the bacterial flora and the relative predominance of each organism must all be considered when assessing the microbiological acceptability of retail 'ready to eat' products.

摘要

在一项实验室间调查中,倾注平板法、表面涂布法、琼脂滴液法和螺旋平板法与表面滴液法同时用于食品中微生物的计数。所有表面计数方法之间取得了良好的一致性,但熔化琼脂法与表面滴液法之间的一致性较差。共检测了1143份在零售点即可食用的食品样本。选择了八种食品:肉馅饼、香肠卷、鲜奶油切片、人造奶油切片、蛋黄酱凉拌卷心菜、肉丸子、肉酱和欧式香肠。本次调查结果表明,可接受活菌数的上限应根据食品种类而有所不同。任何时候均未分离出沙门氏菌。未分离出预期会构成公共卫生危害水平的潜在有害生物。在评估零售“即食”产品的微生物可接受性时,必须考虑有关产品性质、总活菌数、致病、产毒或指示生物的存在与否、细菌菌群谱以及每种生物的相对优势等信息。

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本文引用的文献

1
FOOD HYGIENE STANDARDS IN MANUFACTURE, RETAILING AND CATERING.
R Soc Health J. 1964 Jul-Aug;84:195-201. doi: 10.1177/146642406408400408.
2
Microbiological standards for foods. II.
Lab Pract. 1969 Aug;18(8):839-44 passim.
4
Spiral plate method for bacterial determination.用于细菌测定的螺旋平板法。
Appl Microbiol. 1973 Feb;25(2):244-52. doi: 10.1128/am.25.2.244-252.1973.

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