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食品中微生物的计数:五种方法的比较研究

Enumeration of micro-organisms in food: a comparative study of five methods.

作者信息

Kramer J M, Gilbert R J

出版信息

J Hyg (Lond). 1978 Aug;81(1):151-9. doi: 10.1017/s0022172400053857.

DOI:10.1017/s0022172400053857
PMID:690422
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2129760/
Abstract

Five methods for the enumeration of micro-organisms in food (pour plate, surface spread plate, surface drop, agar droplet, and microdilution) were used in parallel to examine 100 samples selected from a wide range of food products. Statistical analyses of the results showed that the regression and correlation coefficients between the methods were highly significant; the lowest correlation coefficient derived for any pair of methods was 0.979. A variation in count between the methods of less than 0.5 log10 cycles was given by 98% of the samples. Analysis of the operation times and material requirements of each method showed that substantial savings in cost, time, space and support labour were achieved with the microdilution and agar droplet techniques as compared with the conventional pour plate and spread plate methods.

摘要

采用五种食品中微生物计数方法(倾注平板法、表面涂布平板法、表面滴加法、琼脂滴加法和微量稀释法)同时检测从多种食品中选取的100个样本。结果的统计分析表明,这些方法之间的回归系数和相关系数高度显著;任意两种方法之间得出的最低相关系数为0.979。98%的样本在各方法之间的计数差异小于0.5个对数10周期。对各方法的操作时间和材料需求分析表明,与传统的倾注平板法和涂布平板法相比,微量稀释法和琼脂滴加法在成本、时间、空间和辅助人力方面大幅节省。

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本文引用的文献

1
Influence of Subtle Differences in Plating Procedure on Bacterial Counts of Prepared Frozen Foods.装罐程序细微差异对冷冻预制食品细菌计数的影响。
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A rapid, inexpensive bacterial count technique using agar droplets.一种使用琼脂液滴的快速、低成本细菌计数技术。
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Adenosinetriphosphate (ATP) levels in foods contaminated by bacteria.被细菌污染的食品中的三磷酸腺苷(ATP)水平。
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Microtiter method for the evaluation of viable cells in bacterial cultures.
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Comparison of pour and surface plate methods for determination of bacterial counts.倾注法和平板法测定细菌计数的比较。
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6
Spiral plate method for bacterial determination.用于细菌测定的螺旋平板法。
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Radiometric detection of some food-borne bacteria.某些食源细菌的辐射检测
Appl Microbiol. 1972 Oct;24(4):535-9. doi: 10.1128/am.24.4.535-539.1972.
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Technique and apparatus for rapid and inexpensive enumeration of bacteria.快速且低成本的细菌计数技术与设备。
Appl Microbiol. 1972 Jul;24(1):4-7. doi: 10.1128/am.24.1.4-7.1972.
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