Seidlin M, Takiff H E, Smith H A, Hay J, Straus S E
J Med Virol. 1984;13(1):53-61. doi: 10.1002/jmv.1890130107.
Varicella-zoster virus (VZV) infection can be definitively diagnosed by isolation of virus in cell culture, a process that usually takes 7-14 days. In order to facilitate the more rapid detection of this virus, we developed a technique for hybridization of DNA from clinical specimens using an in vitro-labeled mixture of cloned fragments of VZV DNA as a probe. The assay can be completed in 36-48 hr and can be successfully carried out in the range of 10 pg to 10 ng of viral DNA. In analyses of 38 specimens from patients with a clinical diagnosis of VZV infection, the results of viral isolation and this assay were highly concordant. The sensitivity of standard cell culture for detection of VZV was 58%, whereas the sensitivity of the assay was 76%, not significantly different (P = 0.14). The specificity of cell culture was 100%, whereas that of the assay was 94% (P = 0.49). The technique appears to be sensitive, specific, and useful for analyses of tissues and body fluids.
水痘带状疱疹病毒（VZV）感染可通过在细胞培养中分离病毒来明确诊断，这一过程通常需要7至14天。为了更快速地检测这种病毒，我们开发了一种技术，使用体外标记的VZV DNA克隆片段混合物作为探针，对临床标本中的DNA进行杂交。该检测可在36至48小时内完成，并且在10 pg至10 ng的病毒DNA范围内均可成功进行。在对38例临床诊断为VZV感染患者的标本分析中，病毒分离结果与本检测结果高度一致。标准细胞培养检测VZV的灵敏度为58%，而本检测的灵敏度为76%，差异无统计学意义（P = 0.14）。细胞培养的特异性为100%，而本检测的特异性为94%（P = 0.49）。该技术似乎对组织和体液分析具有敏感性、特异性且实用。
