Davies J, Hallworth J A, McLeish P, Randall S, Martin B A, Buchan A, Skinner G R
Vaccine Research Foundation, Lapworth, Warwickshire, UK.
Med Microbiol Immunol. 1994 May;183(2):105-17. doi: 10.1007/BF00277161.
This study describes the properties of an inactivated subunit antigen preparation from varicella-zoster virus (VZV)-infected MRC-5 cells by treatment with detergent and formaldehyde, ultracentrifugation over sucrose and acetone precipitation. The method preserved the antigenicity of VZV proteins and several VZV-specific glycoproteins, while virus DNA was less than 20 pg/250 micrograms protein--a putative vaccine dose. The vaccine was immunogenic in rabbits and stimulated antibodies to the major capsid protein as well as to glycoproteins; an immunoprecipitin was shared with a known immune human serum. The preparation contained no infectious VZV with no evidence of side effects in a rabbit or in five human vaccinees during a follow-up period of 6-10 years.
本研究描述了一种来自水痘带状疱疹病毒（VZV）感染的MRC - 5细胞的灭活亚单位抗原制剂的特性，该制剂通过用去污剂和甲醛处理、在蔗糖上超速离心以及丙酮沉淀来制备。该方法保留了VZV蛋白和几种VZV特异性糖蛋白的抗原性，而病毒DNA含量低于20 pg/250微克蛋白（假定的疫苗剂量）。该疫苗在兔中具有免疫原性，可刺激产生针对主要衣壳蛋白以及糖蛋白的抗体；一种免疫沉淀素与已知的免疫人血清共有。该制剂不含感染性VZV，在6至10年的随访期内，兔和五名人类疫苗接种者均未出现副作用迹象。
