大肠杆菌病毒T1诱导DNA甲基转移酶的证据。
Evidence that Escherichia coli virus T1 induces a DNA methyltransferase.
作者信息
Auer B, Schweiger M
出版信息
J Virol. 1984 Feb;49(2):588-90. doi: 10.1128/JVI.49.2.588-590.1984.
Abstract
DNA of Escherichia coli virus T1 is resistant to MboI cleavage and appears to be heavily methylated. Analysis of methylation by the isoschizomeric restriction enzymes Sau3AI and DpnI revealed that recognition sites for E. coli DNA adenine methylase (dam methylase) are methylated. The same methylation pattern was found for virus T1 DNA grown on an E. coli dam host, indicating a T1-specific DNA methyltransferase.
摘要
大肠杆菌病毒T1的DNA对MboI切割具有抗性，且似乎高度甲基化。通过同裂酶限制酶Sau3AI和DpnI对甲基化进行分析，结果显示大肠杆菌DNA腺嘌呤甲基化酶（dam甲基化酶）的识别位点发生了甲基化。在大肠杆菌dam宿主上生长的病毒T1 DNA也发现了相同的甲基化模式，这表明存在一种T1特异性DNA甲基转移酶。
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