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产生人类T细胞白血病病毒的大鼠淋巴细胞系。I. 生物学和血清学特征

Rat lymphoid cell lines with human T cell leukemia virus production. I. Biological and serological characterization.

作者信息

Tateno M, Kondo N, Itoh T, Chubachi T, Togashi T, Yoshiki T

出版信息

J Exp Med. 1984 Apr 1;159(4):1105-16. doi: 10.1084/jem.159.4.1105.

DOI:10.1084/jem.159.4.1105
PMID:6323614
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2187267/
Abstract

Cocultivation of spleen cells, lymph node cells, and thymocytes of female Wistar-King-Aptekman rats with short-term cultured male adult T cell leukemia (ATL) cells in the presence of 5-bromo-2'-deoxyuridine (BrdUrd) resulted in the establishment of rat lymphoid cell lines, TARS-1, TARL-2, and TART-1. Cytogenetic analysis of the three cell lines showed a female rat karyotype with 42 chromosomes. The surface phenotypes of TARS-1 and TART-1 were those of rat T cells. TARL-2 was only positive for rat Ia and leukocyte common antigens. The cell lines continuously produced a type C retrovirus, human T cell leukemia virus (HTLV) and expressed ATL-associated antigens. TARS-1 and TART-1, but not TARL-2 were transplantable into newborn syngeneic rats and nude mice. These results strongly indicate that HTLV not only immortalizes, but also transforms rat T cells in vitro. Adult rats immunized with either TARS-1 or TARL-2 produced antibodies specific for HTLV. The biochemical analysis of the antigens that reacted with rat sera revealed that they are the two HTLV-specific polypeptides, p24 and p28.

摘要

在5-溴-2'-脱氧尿苷（BrdUrd）存在的情况下，将雌性Wistar-King-Aptekman大鼠的脾细胞、淋巴结细胞和胸腺细胞与短期培养的成年雄性T细胞白血病（ATL）细胞共培养，建立了大鼠淋巴细胞系TARS-1、TARL-2和TART-1。对这三个细胞系的细胞遗传学分析显示为具有42条染色体的雌性大鼠核型。TARS-1和TART-1的表面表型为大鼠T细胞的表型。TARL-2仅对大鼠Ia和白细胞共同抗原呈阳性。这些细胞系持续产生一种C型逆转录病毒，即人类T细胞白血病病毒（HTLV），并表达ATL相关抗原。TARS-1和TART-1可移植到新生同基因大鼠和裸鼠体内，而TARL-2则不能。这些结果有力地表明，HTLV不仅能使大鼠T细胞永生化，还能在体外使其发生转化。用TARS-1或TARL-2免疫成年大鼠可产生针对HTLV的特异性抗体。对与大鼠血清反应的抗原进行生化分析表明，它们是两种HTLV特异性多肽，即p24和p28。

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