Goldblum R M, Dill C W, Albrecht T B, Alford E S, Garza C, Goldman A S
J Pediatr. 1984 Mar;104(3):380-5. doi: 10.1016/s0022-3476(84)81099-9.
Increasing interest in feeding human milk to low-birth-weight infants raises concern about microbial contamination of milk that is pooled or stored. We examined the effect of rapid high-temperature treatment on bacteria and viruses and on the nutritional and immunologic quality of pooled human milk. Growth of endogenous bacteria and infectivity of added cytomegalovirus were undetectable after heating at 72 degrees C for 15 and 5 seconds, respectively. Folic acid and vitamins B1, B2, B6, and C were not affected, whereas bile salt-stimulated lipase was inactivated by these conditions. The concentration of lactoferrin and secretory IgA, and SIgA antibody activity were not changed by heating at 72 degrees C. Lysozyme concentration and enzymatic activity were increased significantly by heat treatment, suggesting that this component may be largely sequestered in milk. Our findings suggest that rapid high-temperature treatment can reduce microbial contamination without destroying the unique nutritional and immunologic qualities of human milk.
越来越多的人关注给低体重婴儿喂食母乳，这引发了对储存或混合母乳微生物污染问题的担忧。我们研究了快速高温处理对细菌、病毒以及混合母乳营养和免疫质量的影响。分别在72摄氏度加热15秒和5秒后，内源性细菌的生长以及添加的巨细胞病毒的传染性均未检测到。叶酸以及维生素B1、B2、B6和C未受影响，而胆汁盐刺激的脂肪酶在这些条件下失活。乳铁蛋白和分泌型IgA的浓度以及分泌型IgA抗体活性在72摄氏度加热时未发生变化。热处理使溶菌酶浓度和酶活性显著增加，这表明该成分在很大程度上可能被隔离在母乳中。我们的研究结果表明，快速高温处理可以减少微生物污染，同时又不会破坏母乳独特的营养和免疫特性。
