水痘带状疱疹病毒基因组的分子克隆及六种限制性内切酶图谱的推导。
Molecular cloning of the varicella-zoster virus genome and derivation of six restriction endonuclease maps.
作者信息
Davison A J, Scott J E
出版信息
J Gen Virol. 1983 Aug;64 (Pt 8):1811-4. doi: 10.1099/0022-1317-64-8-1811.
PMID:6308138
Abstract
KpnI and SstI fragments representing 96% of the varicella-zoster virus genome, including the termini, were cloned in plasmid vector pAT153. The clones were used to derive maps of virion DNA for SstI, KpnI, XhoI, PvuII, EcoRI and SalI by molecular hybridization and restriction endonuclease digestion.
摘要
代表水痘-带状疱疹病毒基因组96%（包括末端）的KpnI和SstI片段被克隆到质粒载体pAT153中。通过分子杂交和限制性内切酶消化，利用这些克隆构建了SstI、KpnI、XhoI、PvuII、EcoRI和SalI的病毒体DNA图谱。
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