禽类疟原虫洛氏疟原虫有少量异质性核糖体RNA基因。

The avian malaria Plasmodium lophurae has a small number of heterogeneous ribosomal RNA genes.

作者信息

Unnasch T R, Wirth D F

出版信息

Nucleic Acids Res. 1983 Dec 10;11(23):8443-59. doi: 10.1093/nar/11.23.8443.

DOI:10.1093/nar/11.23.8443

PMID:6324084

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC326594/

Abstract

The structure and number of the ribosomal RNA (rRNA) genes of the avian malaria parasite Plasmodium lophurae has been examined using Southern blot analysis and recombinant DNA techniques. The ribosomal DNA (rDNA) of P. lophurae has been cloned into the plasmid pBR322, beginning with size-selected populations of Cla I- and Hind III-restricted parasite DNA. The structure of two clones (CL-1 and HA-2) is presented in detail. These two clones together probably represent the entire 17s and 25s coding regions of P. lophurae. Analysis of quantitative genomic Southern blots reveals that there are approximately six rRNA genes per haploid genome, and that the rRNA genes may be divided into four distinct classes by restriction analysis. Examination of the flanking regions of these genes indicates that they are not organized into easily recognizable tandem repeats.

摘要

已使用Southern印迹分析和重组DNA技术对禽类疟原虫洛氏疟原虫的核糖体RNA（rRNA）基因的结构和数量进行了研究。洛氏疟原虫的核糖体DNA（rDNA）已被克隆到质粒pBR322中，起始于经Cla I和Hind III酶切的寄生虫DNA的大小选择群体。详细介绍了两个克隆（CL-1和HA-2）的结构。这两个克隆可能共同代表了洛氏疟原虫的整个17s和25s编码区。对定量基因组Southern印迹的分析表明，每个单倍体基因组大约有六个rRNA基因，并且通过限制性分析，rRNA基因可分为四个不同的类别。对这些基因侧翼区域的检查表明，它们没有组织成易于识别的串联重复序列。