Rotman G, Itin A, Keshet E
Nucleic Acids Res. 1984 Mar 12;12(5):2273-82. doi: 10.1093/nar/12.5.2273.
VL30 genetic elements constitute a murine multicopy gene family that is retrovirus-like, despite the lack of sequence homology with any known retrovirus. Over one hundred copies of VL30 units are dispersed throughout the mouse genome. We report here that the mouse genome also contains 'solo' VL30 long terminal repeats (LTRs). These are structures which contain the LTR detached from the rest of the VL30 sequences. The isolation of solo LTRs from a mouse embryonic gene library with the aid of sub-genomic VL30 probes is described. Direct DNA sequencing established that the solo LTR unit is grossly similar to a standard VL30 LTR and that the LTR is flanked by a 4-base pair duplication. The analogy to the occurrence of solitary LTR units of transposable elements is discussed.
VL30遗传元件构成了一个鼠类多拷贝基因家族，尽管与任何已知逆转录病毒缺乏序列同源性，但它类似逆转录病毒。超过一百个VL30单位拷贝分散在小鼠基因组中。我们在此报告，小鼠基因组中还包含“单独的”VL30长末端重复序列（LTR）。这些结构包含从VL30序列其余部分分离的LTR。本文描述了借助亚基因组VL30探针从小鼠胚胎基因文库中分离单独LTR的方法。直接DNA测序表明，单独的LTR单位与标准VL30 LTR非常相似，并且LTR两侧有一个4碱基对的重复序列。文中讨论了与转座元件单独LTR单位出现情况的类比。
