pcbA突变的图谱定位及突变体的生理学特性
Map location of the pcbA mutation and physiology of the mutant.
作者信息
Bryan S K, Moses R E
出版信息
J Bacteriol. 1984 Apr;158(1):216-21. doi: 10.1128/jb.158.1.216-221.1984.
Abstract
Many temperature-resistant revertants of a polA1 polB polCts (HS432) strain are PolI+ (by either suppression of the polA1 amber allele or intragenic reversion) but remain polCts (contain a temperature-sensitive DNA polymerase III). It appears that DNA replication in such temperature-resistant revertants depends on an extragenic mutation, pcbA, already present in the parent strain and not linked to any of the DNA polymerase loci. This allele allows DNA replication dependent on DNA polymerase I and bypasses a temperature-sensitive DNA polymerase III (polC bypass), so that reversion to PolI+ makes the strain temperature resistant. This pathway of DNA replication also supports phage and plasmid DNA replication. At restrictive temperature, these mutants display a normal response to UV irradiation but show increased sensitivity to the alkylating agent methyl methanesulfonate. We have located pcbA linked to dnaA.
摘要
polA1 polB polCts（HS432）菌株的许多耐温回复突变体是PolI +（通过抑制polA1琥珀色等位基因或基因内回复突变），但仍为polCts（含有温度敏感的DNA聚合酶III）。似乎这种耐温回复突变体中的DNA复制取决于亲本菌株中已存在的一个基因外突变pcbA，且该突变与任何DNA聚合酶基因座均无关联。该等位基因允许依赖DNA聚合酶I的DNA复制，并绕过温度敏感的DNA聚合酶III（polC绕过），因此回复为PolI +可使菌株具有耐温性。这种DNA复制途径也支持噬菌体和质粒DNA复制。在限制温度下，这些突变体对紫外线照射表现出正常反应，但对烷基化剂甲磺酸甲酯的敏感性增加。我们已将pcbA定位到与dnaA连锁的位置。
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