杜克雷嗜血杆菌和淋病奈瑟菌氨苄青霉素抗性质粒的体外复制起源与方向
Origin and direction of in vitro replication of Haemophilus ducreyi and Neisseria gonorrhoeae ampicillin resistance plasmids.
作者信息
McNicol P J, Albritton W L, Ronald A R
出版信息
J Bacteriol. 1984 Apr;158(1):393-5. doi: 10.1128/jb.158.1.393-395.1984.
Abstract
The origin of replication of Haemophilus ducreyi and Neisseria gonorrhoeae ampicillin resistance plasmids was located by cloning BamHI restriction fragments into vector plasmid pAT153 and a derivative plasmid, pAT2. Selection was made for plasmid maintenance in a polA mutant. Direction of replication was determined by in vitro replication of plasmid DNA in the presence of radiolabeled deoxynucleotide.
摘要
通过将杜克雷嗜血杆菌和淋病奈瑟菌氨苄青霉素抗性质粒的BamHI限制性片段克隆到载体质粒pAT153和衍生质粒pAT2中，确定了它们的复制起点。选择在polA突变体中维持质粒。通过在放射性标记的脱氧核苷酸存在下体外复制质粒DNA来确定复制方向。
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