Determination of cytomegalovirus antibodies by enzyme-linked immunosorbent assay (ELISA) in women of fertile period.

Such cytomegalovirus antibodies as the IgG and IgM are investigated in the present work. Investigation was carried out on 301 sera of fertile aged women (between 18 to 35 years of age) under the enzyme-linked immunosorbent assay (ELISA). Only one dilution has been made i.e. 1:40 and the final dilution was worked out from regression graph obtained from a previously titred human positive serum analysis; IgG antibodies were found in 265 cases (88%), appearing IgM antibodies in 4 of them, with the remaining 36 cases showing neither IgG nor IgM antibodies (12%). These results were compared with those obtained in other countries and the importance of the serological diagnosis to this virus was pointed out.