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采用酶联免疫吸附测定法(ELISA)检测育龄期女性的巨细胞病毒抗体。

Determination of cytomegalovirus antibodies by enzyme-linked immunosorbent assay (ELISA) in women of fertile period.

作者信息

Sánchez R T, Ortuño S L, Fariñas M T, Vaillant V S

出版信息

J Hyg Epidemiol Microbiol Immunol. 1984;28(1):85-90.

PMID:6325535
Abstract

Such cytomegalovirus antibodies as the IgG and IgM are investigated in the present work. Investigation was carried out on 301 sera of fertile aged women (between 18 to 35 years of age) under the enzyme-linked immunosorbent assay (ELISA). Only one dilution has been made i.e. 1:40 and the final dilution was worked out from regression graph obtained from a previously titred human positive serum analysis; IgG antibodies were found in 265 cases (88%), appearing IgM antibodies in 4 of them, with the remaining 36 cases showing neither IgG nor IgM antibodies (12%). These results were compared with those obtained in other countries and the importance of the serological diagnosis to this virus was pointed out.

摘要

本研究对巨细胞病毒的IgG和IgM抗体进行了检测。采用酶联免疫吸附测定法(ELISA)对301例18至35岁育龄妇女的血清进行了检测。仅进行了一次稀释,即1:40,并根据先前滴定的人阳性血清分析得到的回归图计算出最终稀释度;265例(88%)检测到IgG抗体,其中4例出现IgM抗体,其余36例既未检测到IgG抗体也未检测到IgM抗体(12%)。将这些结果与其他国家的结果进行了比较,并指出了该病毒血清学诊断的重要性。

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