The effects of relative humidity and temperature on the survival of human rotavirus in faeces.

The effects of relative humidity and temperature on the survival of human rotavirus in a thin layer of faeces on an impervious surface and on absorbent material was investigated using the indirect immunofluorescence technique in LLC-MK2 cells to titrate infectivity. Rotavirus was found to be very stable at low and high relative humidities but not in the medium range of relative humidity. Rotavirus infectivity was lost more rapidly under all humidities at 37 degrees C than at 4 degrees or 20 degrees C.