一个125个碱基对的CEN6 DNA片段足以在酿酒酵母中实现完整的减数分裂和有丝分裂着丝粒功能。
A 125-base-pair CEN6 DNA fragment is sufficient for complete meiotic and mitotic centromere functions in Saccharomyces cerevisiae.
作者信息
Cottarel G, Shero J H, Hieter P, Hegemann J H
机构信息
Institut fuer Mikrobiologie und Molekularbiologie, Justus Liebig Universitaet, Giessen, Federal Republic of Germany.
出版信息
Mol Cell Biol. 1989 Aug;9(8):3342-9. doi: 10.1128/mcb.9.8.3342-3349.1989.
Saccharomyces cerevisiae centromeres contain a conserved region ranging from 111 to 119 base pairs (bp) in length, which is characterized by the three conserved DNA elements CDEI, CDEII, and CDEIII. We isolated a 125-bp CEN6 DNA fragment (named ML CEN6) containing only these conserved elements and assayed it completely separated from its chromosomal context on circular minichromosomes and on a large linear chromosome fragment. The results show that this 125-bp CEN6 DNA fragment is by itself sufficient for complete mitotic and meiotic centromere functions.
酿酒酵母着丝粒包含一个长度在111至119个碱基对（bp）之间的保守区域，其特征在于三个保守的DNA元件CDEI、CDEII和CDEIII。我们分离出了一个仅包含这些保守元件的125 bp的CEN6 DNA片段（命名为ML CEN6），并在环状微型染色体和大型线性染色体片段上对其进行了完全脱离其染色体背景的分析。结果表明，这个125 bp的CEN6 DNA片段自身就足以实现完整的有丝分裂和减数分裂着丝粒功能。
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