两个玉米组蛋白H4基因的基因组结构与核苷酸序列
Genomic organization and nucleotide sequences of two corn histone H4 genes.
作者信息
Philipps G, Chaubet N, Chaboute M E, Ehling M, Gigot C
出版信息
Gene. 1986;42(2):225-9. doi: 10.1016/0378-1119(86)90301-x.
The sea urchin histone H4 gene has been used as a probe to clone two corn histone H4 genes from a lambda gtWES X lambda B corn genomic library. The nucleotide (nt) sequences of both genes showed that the encoded amino acid sequences were identical to that of the H4 of pea and one variant of wheat. The nt sequences of the coding regions showed 92% homology. 5'- and 3'-flanking regions do not show extensive nt sequence analogies. Southern blotting of the EcoRI digested genomic DNA suggests the existence of multiple H4 genes dispersed throughout the genome.
海胆组蛋白H4基因已被用作探针，从λgtWES×λB玉米基因组文库中克隆出两个玉米组蛋白H4基因。两个基因的核苷酸（nt）序列表明，编码的氨基酸序列与豌豆H4和小麦的一个变体相同。编码区的nt序列显示出92%的同源性。5'和3'侧翼区没有显示出广泛的nt序列相似性。用EcoRI消化基因组DNA后的Southern印迹分析表明，基因组中存在多个分散的H4基因。
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