Roussel A F, Chabbert Y A
J Gen Microbiol. 1978 Feb;104(2):269-76. doi: 10.1099/00221287-104-2-269.
Phylogenetic and epidemiological relatedness among transferable plasmids belonging to the IncC, IncM and IncH incompatibility groups has been studied by DNA-DNA filter hydridization. Hybridization was carried out on nitrocellulose microfilters, at low temperature, in formamide and under paraffin oil. The degree of hybridization among plasmids belonging to the IncC and IncM groups supported the conclusions drawn from genetic classification. Studies on relatedness among plasmids belonging to the IncH group allowed their classification into three phylogenetic sub-groups. Comparison of DNA sequences of three plasmids sharing the same genetic properties and isolated from different bacterial species suggested an epidemiological spread of the same plasmid.
通过DNA-DNA滤膜杂交研究了属于IncC、IncM和IncH不相容群的可转移质粒之间的系统发育和流行病学相关性。杂交在硝酸纤维素微滤膜上进行，在低温下，于甲酰胺中并在石蜡油下进行。属于IncC和IncM群的质粒之间的杂交程度支持了从基因分类得出的结论。对属于IncH群的质粒之间相关性的研究使它们能够被分类为三个系统发育亚群。对从不同细菌物种分离出的具有相同遗传特性的三个质粒的DNA序列进行比较，表明同一质粒存在流行病学传播。
